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Nordic Laboratories semi-automated meropenem mic vitek2
Bacterial strains ( n = 27) used in this study and their relevant characteristics
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Pre-purification digests were equally divided into two fractions. Islets <t>were</t> <t>purified</t> using either <t>Ficoll-based</t> or OptiPrep-based density gradients. After 48hr culture, cytokines/chemokines in the supernatant were determined using Bio-Plex in five independent experiments. The concentrations of IFN-γ (A), TNF-α (B), IL-1β (C), IL-6 (D), IL-8 (E), RANTES (F), MCP-1 (G) and MIP-1β (H) were shown. The values were normalized by the protein content of islet preparation in each group. * p<0.05.
Biocoll Separating Solution, supplied by Biocoll GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher dna rna hybrids
Pre-purification digests were equally divided into two fractions. Islets <t>were</t> <t>purified</t> using either <t>Ficoll-based</t> or OptiPrep-based density gradients. After 48hr culture, cytokines/chemokines in the supernatant were determined using Bio-Plex in five independent experiments. The concentrations of IFN-γ (A), TNF-α (B), IL-1β (C), IL-6 (D), IL-8 (E), RANTES (F), MCP-1 (G) and MIP-1β (H) were shown. The values were normalized by the protein content of islet preparation in each group. * p<0.05.
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Pre-purification digests were equally divided into two fractions. Islets <t>were</t> <t>purified</t> using either <t>Ficoll-based</t> or OptiPrep-based density gradients. After 48hr culture, cytokines/chemokines in the supernatant were determined using Bio-Plex in five independent experiments. The concentrations of IFN-γ (A), TNF-α (B), IL-1β (C), IL-6 (D), IL-8 (E), RANTES (F), MCP-1 (G) and MIP-1β (H) were shown. The values were normalized by the protein content of islet preparation in each group. * p<0.05.
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MIMVista corp semi-automatic gradient-based segmentation algorithm
Pre-purification digests were equally divided into two fractions. Islets <t>were</t> <t>purified</t> using either <t>Ficoll-based</t> or OptiPrep-based density gradients. After 48hr culture, cytokines/chemokines in the supernatant were determined using Bio-Plex in five independent experiments. The concentrations of IFN-γ (A), TNF-α (B), IL-1β (C), IL-6 (D), IL-8 (E), RANTES (F), MCP-1 (G) and MIP-1β (H) were shown. The values were normalized by the protein content of islet preparation in each group. * p<0.05.
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Image Search Results


Bacterial strains ( n = 27) used in this study and their relevant characteristics

Journal: Journal of Antimicrobial Chemotherapy

Article Title: Performance of the EUCAST disc diffusion method and two MIC methods in detection of Enterobacteriaceae with reduced susceptibility to meropenem: the NordicAST CPE study

doi: 10.1093/jac/dky276

Figure Lengend Snippet: Bacterial strains ( n = 27) used in this study and their relevant characteristics

Article Snippet: Sixty-one Nordic laboratories delivered data on EUCAST disc diffusion ( n = 61), semi-automated meropenem MIC ( n = 23; VITEK2, n = 20 and Phoenix, n = 3) and gradient meropenem MIC ( n = 58) methods.

Techniques:

Distributions ( n = 1682 <xref ref-type= b ) of meropenem zone diameters (mm) using EUCAST disc diffusion method with BD, MAST or Oxoid discs" width="100%" height="100%">

Journal: Journal of Antimicrobial Chemotherapy

Article Title: Performance of the EUCAST disc diffusion method and two MIC methods in detection of Enterobacteriaceae with reduced susceptibility to meropenem: the NordicAST CPE study

doi: 10.1093/jac/dky276

Figure Lengend Snippet: Distributions ( n = 1682 b ) of meropenem zone diameters (mm) using EUCAST disc diffusion method with BD, MAST or Oxoid discs

Article Snippet: Sixty-one Nordic laboratories delivered data on EUCAST disc diffusion ( n = 61), semi-automated meropenem MIC ( n = 23; VITEK2, n = 20 and Phoenix, n = 3) and gradient meropenem MIC ( n = 58) methods.

Techniques: Diffusion-based Assay

Agreement (%) between  meropenem   MIC  determined by BMD and disc diffusion, semi-automated susceptibility testing or gradient tests and corresponding CA (%) in clinical SIR categorization

Journal: Journal of Antimicrobial Chemotherapy

Article Title: Performance of the EUCAST disc diffusion method and two MIC methods in detection of Enterobacteriaceae with reduced susceptibility to meropenem: the NordicAST CPE study

doi: 10.1093/jac/dky276

Figure Lengend Snippet: Agreement (%) between meropenem MIC determined by BMD and disc diffusion, semi-automated susceptibility testing or gradient tests and corresponding CA (%) in clinical SIR categorization

Article Snippet: Sixty-one Nordic laboratories delivered data on EUCAST disc diffusion ( n = 61), semi-automated meropenem MIC ( n = 23; VITEK2, n = 20 and Phoenix, n = 3) and gradient meropenem MIC ( n = 58) methods.

Techniques: Diffusion-based Assay

Reporting potential carbapenemase production from the laboratories ( n = 59) based on the use of different  meropenem  zone diameter screening cut-off criteria; the recommended ( <27 mm), <25 mm and <22 mm (clinical breakpoint)

Journal: Journal of Antimicrobial Chemotherapy

Article Title: Performance of the EUCAST disc diffusion method and two MIC methods in detection of Enterobacteriaceae with reduced susceptibility to meropenem: the NordicAST CPE study

doi: 10.1093/jac/dky276

Figure Lengend Snippet: Reporting potential carbapenemase production from the laboratories ( n = 59) based on the use of different meropenem zone diameter screening cut-off criteria; the recommended ( <27 mm), <25 mm and <22 mm (clinical breakpoint)

Article Snippet: Sixty-one Nordic laboratories delivered data on EUCAST disc diffusion ( n = 61), semi-automated meropenem MIC ( n = 23; VITEK2, n = 20 and Phoenix, n = 3) and gradient meropenem MIC ( n = 58) methods.

Techniques:

Pre-purification digests were equally divided into two fractions. Islets were purified using either Ficoll-based or OptiPrep-based density gradients. After 48hr culture, cytokines/chemokines in the supernatant were determined using Bio-Plex in five independent experiments. The concentrations of IFN-γ (A), TNF-α (B), IL-1β (C), IL-6 (D), IL-8 (E), RANTES (F), MCP-1 (G) and MIP-1β (H) were shown. The values were normalized by the protein content of islet preparation in each group. * p<0.05.

Journal: Cell transplantation

Article Title: Anti-proinflammatory Effects of Iodixanol (OptiPrep)-Based Density Gradient Purification on Human Islet Preparations

doi: 10.3727/096368910X516600

Figure Lengend Snippet: Pre-purification digests were equally divided into two fractions. Islets were purified using either Ficoll-based or OptiPrep-based density gradients. After 48hr culture, cytokines/chemokines in the supernatant were determined using Bio-Plex in five independent experiments. The concentrations of IFN-γ (A), TNF-α (B), IL-1β (C), IL-6 (D), IL-8 (E), RANTES (F), MCP-1 (G) and MIP-1β (H) were shown. The values were normalized by the protein content of islet preparation in each group. * p<0.05.

Article Snippet: Islets were purified using either continuous Ficoll (Biocoll Separating Solution, density 1.108 g/mL, osmolality 320–373 mOsm/kg/H 2 O; Biochrom KG, Berlin, Germany) -based or OptiPrep (density 1.320 g/mL, osmolality 170 mOsm/kg/H 2 O; Axis- Shield, Oslo, Norway) -based density gradients, with a semi-automated cell processor (Cobe 2991; COBE Laboratories, Inc. Lakewood, CO) as described previously with modifications ( 4 , 6 ).

Techniques: Purification